치아재식후 치유과정에서 세포부착성 glycoproteins의 분포에 관한 면역조직화학적 연구
IMMUNOHISTOCHEMICAL DISTRIBUTION OF ADHESION GLYCOPROTEIN DURING REPAIR OF RAT REPLANTATION MODEL
소속 상세정보
신제원/Je Won Shin
KMID : 0355819960200020181
Abstract
-Abstract-
Periodontal healing requires the formation of new periodontal tissues comprised of
various extracellular components. The present study investigated the formation and
distribution of the fibronectin (FN), a major adhesive glycoprotein. Rats weighing 130∼
150 gm were fed 0.4% β-aminopropionitrile for 5 days to achieve gentle tooth
extraction. The maxillary first molars were extracted under anesthesia with
pentobarbital, washed in sterile distilled water, treated with bacterial collagenase to
digest collagen fibers on the root surfaces. After washing in water overnight, the mesial
root surfaces were demineralized by application of citric acid, washed, dried and stored
at 4℃.
Immediately after tooth extraction and bleeding control, the treated molars extracted
previously from other rats were reimplanted.
Ten animals each were sacrificed 45 min, 1, 3, 6, 10 days after reimplantation of teeth
by intracardiac perfusion with 4% paraformaldehyde. The repalnted molars and
surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin.
Immunostaining of the FN was achieved by the avidin-biotin complex method. The
results as follows;
1. The control group showed gradual healing from 1 day and complete regeneration of
the periodontium at 10 days.
2. At later healing stage, intense staining for FN and type I collagen were observed in
the newly formed connective tissues, granulation tissue, especially around blood vessels
as well as infalmmatory cells.
3. At later healing stage, FN and type I collagen were expressed later in the periodontal
ligament, and newly formed connective tissue, whereas bone sialoprotein, osteopontin,
and osteonectin were expressed its attachment site to the root surface.
4. Vitronectin was expressed evenly in newly formed periodontal ligament.
5. Strong immunolabeling for bone sialoprotein, osteopontin, and osteonectin were found
in cementoblasts, osteoblasts, and new bone and cementum, whereas weaken staining of
FN and type I collagen was observed on the cementum and alveolar bone.
6. A specific staining for osteonectin in newly formed periodontal ligament is very weak.
These results suggest that the healing after replantation is achieved through a series
of cell differentiation and formation of special glycoproteins.
키워드
원문 및 링크아웃 정보
등재저널 정보